When you are doing dilutions the larger number is always thetotal final volume. The smaller number is the amount of thesolute. The larger number minus the smaller number is the amount ofthe solvent. So a 1:5 or a 1 IN 5 dilution would be 1 partsolute and 4 parts solvent to come to a total volume of 5.

• Alsee 5 3 Serial Dilutions Pdf

Serial Dilution Technique: This technique is useful for obtaining countable numbers of bacterial colonies by diluting a highly concentrated culture. The protocol described here is for serial 1:10 dilutions and can be modified for any dilution series desired. Generally, the last 3 dilutions are plated in duplicate or triplicate. Dilutions: Explanations and Examples of Common Methods. There are many ways of expressing concentrations and dilution. The following is a brief explanation of some ways of calculating dilutions that are common in biological science and often used at Quansys Biosciences.

When referencing ratios in mathematics 1:5 means 1 TO 5.The ratio is relative to the two items. For every 1 of the firstyou have 5 of the second.

It is critical if you are making dilutions to understand thedifference as the two explanations are not interchangeable.

Alsee 5 3 Serial Dilutions Pdf

When you are doing dilutions the larger number is always thetotal final volume. The smaller number is the amount of thesolute. The larger number minus the smaller number is the amount ofthe solvent. So a 1:5 or a 1 IN 5 dilution would be 1 partsolute and 4 parts solvent to come to a total volume of 5.

When referencing ratios in mathematics 1:5 means 1 TO 5.The ratio is relative to the two items. For every 1 of the firstyou have 5 of the second.

It is critical if you are making dilutions to understand thedifference as the two explanations are not interchangeable.